Abstract
Radiolabeled anthranilic diamide insecticide [N-C(3)H(3)]chlorantraniliprole was synthesized at high specific activity. It was compared with phthalic diamide insecticide flubendiamide and [(3)H]ryanodine in radioligand binding studies with house fly muscle membranes to provide the first direct evidence with a native insect ryanodine receptor that the major anthranilic and phthalic diamide insecticides bind at different allosterically coupled sites, i.e., there are three distinct Ca(2+)-release channel targets for insecticide action.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Benzamides / chemistry
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Benzamides / metabolism*
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Binding Sites
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Calcium / metabolism
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Carbon / chemistry
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Insect Proteins / chemistry
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Insect Proteins / metabolism*
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Insecta
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Insecticides / chemistry
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Insecticides / metabolism*
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Nitrogen / chemistry
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Protein Binding
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Ryanodine / chemistry
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Ryanodine / metabolism*
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Ryanodine Receptor Calcium Release Channel / chemistry
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Ryanodine Receptor Calcium Release Channel / metabolism*
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Sulfones / chemistry
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Sulfones / metabolism*
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Tritium / chemistry
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ortho-Aminobenzoates / chemistry
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ortho-Aminobenzoates / metabolism*
Substances
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Benzamides
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Insect Proteins
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Insecticides
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Ryanodine Receptor Calcium Release Channel
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Sulfones
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ortho-Aminobenzoates
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Tritium
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Ryanodine
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chlorantranilipole
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Carbon
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flubendiamide
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Nitrogen
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Calcium