Abstract
A group of nine Gram-negative staining, facultatively anaerobic bacterial strains isolated from native oak trees displaying symptoms of acute oak decline (AOD) in the UK were investigated using a polyphasic approach. 16S rRNA gene sequencing and phylogenetic analysis revealed that these isolates form a distinct lineage within the genus Brenneria, family Enterobacteriaceae, and are most closely related to Brenneria rubrifaciens (97.6 % sequence similarity to the type strain). Multilocus sequence analysis based on four housekeeping genes (gyrB, rpoB, infB and atpD) confirmed their position within the genus Brenneria, while DNA-DNA hybridization indicated that the isolates belong to a single taxon. The isolates can be differentiated phenotypically from their closest phylogenetic neighbours. The phylogenetic and phenotypic data demonstrate that these isolates from oak with symptoms of AOD represent a novel species in the genus Brenneria, for which the name Brenneria goodwinii sp. nov. (type strain FRB 141(T) = R-43656(T) = BCC 845(T) = LMG 26270(T) = NCPPB 4484(T)) is proposed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Typing Techniques
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DNA, Bacterial / genetics
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Enterobacteriaceae / classification*
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Enterobacteriaceae / genetics
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Enterobacteriaceae / isolation & purification
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Fatty Acids / analysis
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Molecular Sequence Data
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Multilocus Sequence Typing
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Nucleic Acid Hybridization
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Phenotype
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Phylogeny*
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Plant Diseases / microbiology*
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Quercus / microbiology*
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
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United Kingdom
Substances
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DNA, Bacterial
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Fatty Acids
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RNA, Ribosomal, 16S
Associated data
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GENBANK/JN544202
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GENBANK/JN544203
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GENBANK/JN544204
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GENBANK/JN544205
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GENBANK/JN544206
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GENBANK/JN544207
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GENBANK/JN544208
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GENBANK/JN544209
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GENBANK/JN544210
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GENBANK/JN544211
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GENBANK/JN544212
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GENBANK/JN544213
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GENBANK/JN544214
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GENBANK/JN544215
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GENBANK/JN544216
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GENBANK/JN544217
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GENBANK/JN544218
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GENBANK/JN544219
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GENBANK/JN544220
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GENBANK/JN544221
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GENBANK/JN544222
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GENBANK/JN544223
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GENBANK/JN544224
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GENBANK/JN544225
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GENBANK/JN544226
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GENBANK/JN544227
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GENBANK/JN544232
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GENBANK/JN544233
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GENBANK/JN544234
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GENBANK/JN544235
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GENBANK/JN544236
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GENBANK/JN544237
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GENBANK/JN544238
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GENBANK/JN544239
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GENBANK/JN544240