Abstract
Fagus silvatica L. inoculated or not with the ectomycorrhizal fungusLaccaria laccata and/or a phosphate-solubilizing rhizobacterium,Agrobacterium radiobacter, was cultivated in a greenhouse in lysimeter cylinders containing rock phosphate and a mica (phlogopite) as the only sources of P, Fe, Mg and Al. After two years, beech dry matter and P, Mg, Fe and K uptake were higher for plants inoculated—either withAgrobacterium radiobacter or withLaccaria laccata, but not for those plants inoculated with both micro-organisms—than for uninoculated plants. Mineral element mobilization from rock phosphate and from phlogopite was also greater when plants were inoculated with the bacteria or with the ectomycorrhizal fungi. Such mineral element dissolution can be related to the increased root growth but also to the amount of organic acids released in the rhizosphere. The absence of significant effects of dual inoculation suggested competition between micro-organisms.
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References
Atlas R M 1982 Enumeration and estimation of microbial biomass.In Experimental Microbial Ecology. Eds. R G Burns and J H Slater. pp 84–102. Blackwell Scientific Publications. London.
Azcon R, Barea J M and Hayman D S 1976 Utilization of rock phosphate in alkaline soils by plants inoculated with mycorrhizal fungi and phosphate-solubilizing bacteria. Soil Biol. Biochem. 8, 135–138.
Azcon-Aguilar C, Gianinazzi-Pearson V, Fardeau J C and Gianinazzi S 1986 Effect of vesicular-arbuscular mycorrhizal fungi and phosphate-solubilizing bacteria on growth and nutrition of soybean in a neutral-calcareous soil amended with32P−45Ca-tricalcium phosphate. Plant and Soil 96, 3–15.
Babenko Yu S, Tyrygina G I, Grigoryev E F, Dolgikh L M and Borisova T I 1984 Biological activity and physiologo-biochemical properties of bacteria dissolving phosphates. Mikrobiologiya 53, 533–539.
Bagyaraj D J and Menge J A 1978 Interaction between a VA mycorrhiza and Azotobacter and their effects on rhizosphere microflora and plant growth. New Phytol. 80, 567–573.
Banik S and Dey BK 1982 Available phosphate content of an alluvial soil as influenced by inoculation of some isolated phosphate-solubilizing microorganisms. Plant and Soil 69, 353–364.
Barea J M, Azcon R and Hayman D S 1975 Possible synergistic interactions between endogone and phosphate-solubilizing bacteria in low-phosphate soilsIn Endomycorrhizas. Eds. Sanders, Mosse and Tinker. pp. 409–417. Academic Press, London.
Barea J M, Azcon R and Azcon-Aguilar 1983 Interactions between phosphate-solubiliziing bacteria and VA mycorrhiza to improve the utilization of rock phosphate by plants in non acidic soils.In Proceedings of 3rd Intern. Cong. on Phosphorous Compounds. Oct. 1983. pp 127–144. Brussels.
Berthelin J 1983 Microbial weathering processes.In Microbial Geochemistry. Ed. W E Krumbein. pp 223–262. Blackwell Scientific Publications, London.
Berthelin J 1988 Microbial weathering processes in natural environments.In Physical and Chemical Weathering in Geochemical Cycles. Eds. A Lerman and M Meybeck. pp 33–59. Reidel, Dordrecht.
Berthelin J and Leyval C 1982 Ability of symbiotic and non symbiotic rhizospheric microflora of maize (Zea mays) to weather micas and to promote plant growth and plant nutrition. Plant and Soil 68, 369–377.
Bowen G D and Theodorou C 1979 Interactions between bacteria and ectomycorrhizal fungi. Soil Biol. Biochem. 11, 119–126.
Brown M E and Carr G H 1979 Effects on plant growth of mixed inocula of VA endophytes and root micro-organisms. Rothamsted Experimental Station Report for 1979, Part 1, 189.
Brown M E and Carr G H 1984 Interactions betweenAzotobacter chroococcum and vesicular-arbuscular mycorrhiza and their effect on plant growth. J. Appl. Bacteriol. 56, 429–437.
Chakly M and Berthelin J 1982 Rôle d'une ectomycorhizePisolithus tinctorius-Pinus caribea et d'une bactérie rhizosphérique sur la mobilisation du phosphore de phosphates minéraux et organiques insolubles.In Les Mycorhizes: Biologie et Utilisation. Eds. Les Colloques de l'INRA 13. pp. 215–220. INRA Publication.
El Gibaly M H, El Reweiny F A, Abdel Nasser M and El Dathory T A 1977 Studies on phosphate-solubilizing bacteria in soil and rhizosphere of different plants. III. Inoculation with phosphorous-dissolving micro-organisms. Bacteriol. Parasitenkd. Infektionskr. Hyg. II 132, 245–250.
Gianinazzi S and Gianinazzi-Pearson V 1983 The physiology of vesicular-arbuscular mycorrhizal roots. Plant and Soil 71, 197–209.
Harley J L 1969 The Biology of Mycorrhiza. 2nd Edition. Vol. 1. Leonard Hill Publ., London, 334 p.
Hayman D S and Mosse B 1972 Plant growth responses to vesicular-arbuscular mycorrhiza. III. Increase uptake of labile P from soil. New Phytol. 71, 41–47.
Ho I 1988 Interaction between VA-mycorrhizal fungus and Azotobacter and their combined effects on growth of tall fescue. Plant and Soil 105, 291–293.
Kidd F A Wullschleger S D, Dawley K and Reid C P P 1982 Use of gentamicin in axenic culturing of ectomycorrhizal plants. Appl. Environ. Microb. 44, 506–508.
Kucey R M N 1987 Increased phosphorous uptake by wheat and field beans inoculated with a phosphorous-solubilizingPenicillium bilaji strain and with vesicular-arbuscular mycorrhizal fungi. Appl. Environ. Microbiol. 53, 2699–2703.
Laheurte F and Berthelin J 1986 Interactions between endomycorrhizas and phosphate solubilizing bacteria: Effects on nutrition and growth of maize.In Physiological and Genetical Aspects of Mycorrhizae. Eds. V Gianinazzi-Pearson and S Gianinazzi. pp 425–429. INRA, Paris.
Laheurte F and Berthelin J 1988 Effect of a phosphate solubilizing bacteria on maize growth and root exudation over four level of labile phosphorus. Plant and Soil 105, 11–17.
Leyval C 1988 Interactions Bactéries-Mycorhizes dans la Rhizosphère du Pin Sylvestre et du Hêtre: Incidences sur l'Exsudation Rhizosphérique et l'Altération des Minéraux, Thèse de Doctorat d'Etat, Université de Nancy I, France.
Leyval C 1989 The interactions between micro-organisms, mineral and forest tree roots: lysimeters in a greenhouse.In Fields Methods in Terrestrial Ecosystem Nutrient Cycling. Grange-over-Sand, U.K. (In press).
Leyval C and Berthelin J 1986 Comparison between the utilization of phosphorus from insoluble mineral phosphates by ectomycorrhizal fungi and rhizobacteria.In Physiological and Genetical Aspects of Mycorrhizae. Eds. V Gianinazzi-Pearson and S Gianinazzi pp 340–345. INRA, Paris.
Leyval C and Berthelin J 1988 Interactions between ectomycorrhizal fungi and phosphate-solubilizing bacteria: Phosphorous mobilization from different inorganic phosphates.In Current Perspectives in Environmental Biogeochemistry. Eds. G Giovannozzi-Sermanni and P Nannipieri. pp 257–270. Rome, Italy.
Li C Y and Hung L L 1987 Nitrogen-fixing (acetylene-reducing) bacteria associated with ectomycorrhizae of Douglas-fir. Plant and Soil 98, 425–428.
Manjunath A, Mohan R and Bagyaraj D J 1981 Interaction betweenBeijerinckia mobilis, Aspergillus niger andGlomus fasciculatus and their effects on growth of onion. New Phytol. 87, 723–727.
Pachlewski R and Pachlewska J 1974 Studies on symbiotic properties of mycorrhizal fungi of pinePinus sylvestris L. with the aid of methods of mycorrhizal synthesis in pure culture in agar. Forest Res. Inst Warsaw.
Pareek R P and Gaur A C 1973 Release of phosphate from tricalcium and rock phosphate by organic acids. Curr. Sci. 42 278–279.
Piccini D and Azcon R 1987 Effect of phosphate-solubilizing bacteria and vesicular-arbuscular mycorrhizal fungi on the utilization of Bayovar rock phosphate by alfalfa plants using a sand-vermiculite medium. Plant and Soil 101, 45–50.
Plassard C, Mousain D and Salsac L 1983 Dosage de la chitine sur des ectomycorhizes de pin maritime (Pinus pinaster) àPisolithus tinctorius: Évaluation de la masse mycelienne et de la mycorhization. Can. J. Bot. 61 692–699.
Raj J, Bagyaraj D J and Manjunath A 1980 Influence of soil inoculation with vesicular-arbuscular mycorrhizae and a phosphate-dissolving bacterium on plant growth and32P uptake. Soil Biol. Biochem. 13, 105–108.
Renaut J, Marion-Espinasse H and Lacrampe J L 1986 Influence des champignons endomycorhizogènes vésiculoarbusculaires et deRhizobium leguminosarum sur la croissance d'une gesse pérenne:Lathyrus latifolius L. Rev. Ecol. Biol. Sol 23, 249–257.
Strzelczyk E and Kampert M 1987 Growth of mycorrhizal fungi cultured with bacteria. Angew. Bot. 61, 157–162.
Strzelczyk E and Pokojska-Burdziej A 1984 Production of auxins and gibberellin-like substances by mycorrhizal fungi, bacteria and actinomycetes isolated from soil and the mycorrhizosphere of pine (Pinus sylvestris L.). Plant and Soil 81, 185–194.
Subba-Rao N S, Tilak K V B R and Singh C S 1985 Effect of combined inoculation ofAzospirillum brasilense and vesicular-arbuscular mycorrhiza on pearl millet (Pennisetum americanum). Plant and Soil 84, 283–292.
Swift M J 1973 The estimation of mycelium biomass by determination of the hexosamine content of wood tissue decayed by fungi. Soil Biol. Biochem. 5, 321–332.
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Leyval, C., Berthelin, J. Interactions betweenLaccaria laccata, Agrobacterium radiobacter and beech roots: Influence on P, K, Mg, and Fe mobilization from minerals and plant growth. Plant Soil 117, 103–110 (1989). https://doi.org/10.1007/BF02206262
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DOI: https://doi.org/10.1007/BF02206262