Table 3.

Results of the in-field LAMP assays and corresponding laboratory verification to detect Raffaelea lauricola from wood tissue samples collected and tested in-field at sites with laurel wilt disease in the southeastern US.

State	Date	Site name	Host species	Sample number	In-field LAMP	Fungal isolation^{^a}	Further assessment^{^b}
Georgia	18 Nov 2019	Di-Lane Wildlife Management Area	Redbay	1	−	−
				2	+	+
				3	+	+
				4	+	+
		Mitchell Road	Sassafras	1	−	−
				2	+	−^{^a}	+
				3	+	+
				4	+	−^{^a}	+
Kentucky	11 Mar 2020	Fort Campbell	Sassafras	1	+	+
				2	+
				3	+	+
				4	+	+
				5	+	+
				6	+	+
North Carolina	04 Mar 2020	Mount Olive	Redbay	1	+	+
				2	+	+
				3	+	+
				4	+	+
				5	−	+^{^a}	+
				6	−	+^{^a}	+
South Carolina	26 Nov 2019	Sesquicentennial State Park	Redbay	1	−	+^{^a}	+
				2	+	+
				3	−	−
				4	+	+
				5	+	+
			Sassafras	1	+	−^{^a}	+
				2	+	+
				3	+	+
Tennessee	23 Jan 2020	Cherokee Park	Sassafras	1	+	+
				2	+	+
				3	+	+
				4	+	+
				5	+	+
				6	+	+

↵a Discording results between in-field LAMP assays and fungal isolations, which prompted a further assessment of the sample.
↵b Samples that were positive in-field but negative when plated were further assessed by high quality DNA extraction followed by a LAMP assay on benchtop equipment; samples that were negative in-field but positive when plated were further assessed by a crude DNA extraction followed by a LAMP assay on a portable device.