Multiplex polymerase chain reaction (PCR) primer combinations, diagnostic purposes, and positively tested species.
| Multiplex PCR | Primers combinationz | Taxon-specific amplicon size/taxon identified | Positively tested fungal species | |
|---|---|---|---|---|
| Forward | Reverse | |||
| M1 | ITS1-F | ITS4 | 600–850 bp/fungi | |
| Gano2R | 226–228 bp/Ganoderma spp. | G. adspersum, G. applanatum, G. lucidum, G. resinaceum | ||
| F115 | Hyme2R | 111 bp/Inonotus spp.–Phellinus spp. | I. andersonii, I. dryophilus, I. dryadeus, I. hispidus, I. radiatus, I. tamaricis, P. gilvus, P. ign, P. pini, P. punctatus, P. robustus, P. torulosus, P. tremulae, P. tuberculosas | |
| M2 | ITS3 | Armi2R | 185 bp/Armillaria spp. | A. gallica, A. mellea, A. nabsnona |
| 25sF | LaetR | 146 bp/L. sulphureus | L. sulphureus | |
| Pleu2R | 158 bp/Pleurotus spp. | P. ostreatus, P. pulmonarius | ||
| Heri2R | 200 bp/Hericium spp. | H. coralloides, H. erinaceum | ||
| M3 | ITS3 | PerR | 152 bp/P. fraxinea | P. fraxinea |
| Schi2R | 190 bp/Schizophyllum spp. | S. commune, S. radiatum | ||
| Ste2R | 231–236 bp/Stereum spp. | S. hirsutum, S. rugosum, S. sanguinolentum | ||
| Ustu2R | 260 bp/U. deusta | U. deusta | ||
| MS1 | TraR | 220 bp/Trametes spp. | T. cervina, T. versicolor, T. zonatella | |
| Mgano | ITS1-F | GadR | 211 bp/G. adspersum | G. adspersum |
| GapR | 200 bp/G. applanatum | G. applanatum | ||
| GlR | 193 bp/European G. lucidum | G. lucidum (from Europe) | ||
| GrR | 178 bp/G. resinaceum | G. resinaceum, G. lucidum (from North America) | ||
| Mhyme | 25sF | FomR | 258 bp/Fomitiporia | P. punctatus, P. robustus |
| FuscR | 225 bp/Fuscoporia | P. gilvus, P. torulosus | ||
| IdryaR | 254 bp/Pseudoinonotus | I. dryadeus | ||
| InocuR | 265 bp/Inocutis | I. dryophilus | ||
| InssR | 214 bp/Inonotus s. s. | I. andersonii, I. hispidus | ||
| PhssR | 173 bp/Phellinus s.s. | P. igniarius, P. tremulae, P. tuberculosus | ||
↵zPrimers are reported in Guglielmo et al. (2007, 2008). Primer for Ustulina deusta is reported in this study.