@article {Percival89, author = {Glynn C. Percival and Ian P. Keary and Kelly Noviss}, title = {The Potential of a Chlorophyll Content SPAD Meter to Quantify Nutrient Stress in Foliar Tissue of Sycamore (Acer pseudoplatanus), English Oak (Quercus robur), and European Beech (Fagus sylvatica)}, volume = {34}, number = {2}, pages = {89--100}, year = {2008}, doi = {10.48044/jauf.2008.012}, publisher = {Arboriculture \& Urban Forestry (AUF)}, abstract = {The chlorophyll content (or SPAD meter) is a simple, portable diagnostic tool that measures the greenness or relative chlorophyll content of leaves. Compared with the traditional destructive methods of chlorophyll extraction, the use of this equipment saves time, space, and resources. The objective of this study was to establish a correlation between the leaf photosynthetic pigment content (chlorophylls, carotenoids) extracted in aqueous acetone, total leaf nitrogen (N) content, and chlorophyll fluorescence Fv/Fm values with the SPAD-502 readings in sycamore (Acer pseudoplatanus), beech (Fagus sylvatica), and English oak (Quercus robur) leaves displaying visual symptoms of N deficiency. In addition, this study aimed to determine a critical foliar N content below which a reduction in photosynthetic efficiency occurs. Irrespective of species, high correlations were recorded between SPAD readings, total leaf chlorophyll and carotenoid content, foliar N content, and leaf photosynthetic efficiency as measured by chlorophyll fluorescence Fv/Fm values; however, a poor correlation between SPAD values and total chlorophyll: carotenoid ratios was obtained. In the case of Acer pseudoplatanus, Fagus sylvatica, and Quercus robur, SPAD readings lower than 25 indicated impairment of leaf photosynthetic process that in turn were correlated with a foliar N content less than 1.5\%, a value associated with a critical N deficiency. Results of this study indicate that the chlorophyll content SPAD-502 m potentially offers a useful nondestructive, handheld system to aid in the evaluation of tree health. However, users should be aware of the limitations of this system. Consistency in sample collection and seasonal timing may necessitate species and cultivar calibration equations to correlate SPAD values with reductions in tree vitality.}, issn = {1935-5297}, URL = {https://auf.isa-arbor.com/content/34/2/89}, eprint = {https://auf.isa-arbor.com/content/34/2/89.full.pdf}, journal = {Arboriculture \& Urban Forestry (AUF)} }