TY - JOUR T1 - Microprojectile and <em>Agrobacterium</em>-Mediated Transformation of Pioneer ELM JF - Arboriculture &amp; Urban Forestry (AUF) SP - 34 LP - 37 DO - 10.48044/jauf.1991.011 VL - 17 IS - 2 AU - Mark G. Bolyard AU - Ravindra K. Hajela AU - Mariam B. Sticklen Y1 - 1991/02/01 UR - http://auf.isa-arbor.com/content/17/2/34.abstract N2 - We have transformed internode sections of the hybrid elm cultivar “Pioneer” to assess the possibilities of introducing significant genes into elms. Foreign DNA encoding beta-glucuronidase (GUS) was introduced into internode sections using microprojectile- or Agrobacterium tumefaciens-mediated transformation. Positive signals were detected by Southern analysis. Also, some enzymatic activity was detected in Agrobacterium transformed cultures using a fluorogenic assay. ER -