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Research ArticleArticles

Effect of the Growth Regulator Paclobutrazol on Growth of the Bacterial Pathogen Xylella fastidiosa

Darren A. DeStefano, Arv P. Grybauskas, James L. Sherald, Bahram Momen, Qi Huang and Joe H. Sullivan
Arboriculture & Urban Forestry (AUF) July 2007, 33 (4) 246-252; DOI: https://doi.org/10.48044/jauf.2007.028
Darren A. DeStefano
Darren A. DeStefano, Department of Natural Resource Sciences and Landscape Architecture, University of Maryland, College Park, MD 20742-4452, U.S.
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Arv P. Grybauskas
Arv P. Grybauskas, Department of Natural Resource Sciences and Landscape Architecture, University of Maryland, College Park, MD 20742-4452, U.S.
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James L. Sherald
James L. Sherald, Chief, Center for Urban Ecology, National Park Service, 4598 MacArthur Blvd., NW, Washington, DC 20007, U.S.
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Bahram Momen
Bahram Momen, Department of Natural Resource Sciences and Landscape Architecture, University of Maryland, College Park, MD 20742-4452, U.S.
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Qi Huang
Qi Huang, Research Plant Pathologist, Floral and Nursery Plants Research Unit, U.S. National Arboretum, U.S. Department of Agriculture, 10300 Baltimore Avenue, Beltsville, MD 20705, U.S.
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Joe H. Sullivan
Joe H. Sullivan (corresponding author), Department of Natural Resource Sciences and Landscape Architecture, University of Maryland, College Park, MD 20742-4452, U.S.,
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  • For correspondence: [email protected]
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  • Figure 1.
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    Figure 1.

    The relationship between absorbance of a periwinkle wilt broth sample measured by a spectrophotometer and amount of Xylella fastidiosa colony-forming units (CFUs) within the sample as determined by dilution plating.

  • Figure 2.
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    Figure 2.

    Growth curve of Xylella fastidiosa isolate elm (top) and grape (bottom) in periwinkle wilt (PW) broth supplemented with chemical treatments of either oxytetracycline (antibiotic) or paclobutrazol (PBZ) measured over 10 days with a spectrophotometer. Absorbance readings were converted to colony-forming units (CFUs) using the regression equation (Figure 1) determined for this study. Means and associated standard errors are plotted.

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    Figure 3.

    Growth curve of Xylella fastidiosa isolate elm (left) and grape (right) in periwinkle wilt (PW) broth supplemented with chemical treatments of oxytetracycline (antibiotic) and paclobutrazol (PBZ) measured over 5 days. Colony-forming units (CFUs) were determined by dilution plating. Means and associated standard errors are plotted.

Tables

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    Table 1.

    Shade tree hosts of Xylella fastidiosa.

    Scientific nameCommon name
    Acer negundoBoxelder
    A. rubrumRed maple
    A. saccharumSugar maple
    Cornus floridaFlowering dogwood
    Celtis occidentalisHackberry
    Liquidambar styracifluaSweetgum
    Morus albaWhite mulberry
    Platanus occidentalisAmerican sycamore
    P. x acerifoliaLondon plane
    Quercus albaWhite oak
    Q. bicolorSwamp white oak
    Q. coccineaScarlet oak
    Q. falcateSouthern red oak
    Q. imbricariaShingle oak
    Q. incanaBluejack oak
    Q. laevisTurkey oak
    Q. laurifoliaLaurel oak
    Q. macrocarpaBurr oak
    Q. nigraWater oak
    Q. palustrisPin oak
    Q. phellosWillow oak
    Q. prinusChestnut oak
    Q. rubraNorthern red oak
    Q. shumardiiShumard oak
    Q. stellataPost oak
    Q. velutinaBlack oak
    Q. virginianaLive oak
    Ulmus americanaAmerican elm
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    Table 2.

    Growth of Xylella fastidiosa isolates from elm and grape grown in periwinkle wilt broth supplemented with either oxytetracycline (antibiotic) or paclobutrazol (PBZ).z

    Treatment and doseMean log CFU/mL−1
    ElmGrape
    Oxytetracycline 50 μg/mL−14.97 a4.95 a
    PBZ 200 μg/mL−15.00 a4.99 a
    PBZ 20 μg/mL−17.23 b8.31 b
    PBZ 2 μg/mL−17.18 b8.27 b
    Periwinkle wilt media7.38 b8.61 b
    • ↵zGrowth was measured with a spectrophotometer and converted to colony-forming units (CFUs) using a regression equation (Figure 1).

    • Values are means of three replicates after 10 days of incubation.

    • Means in each column with identical letters are not significantly different at the 0.05 level by Tukey test SED = 0.028 (elm), 0.52 (grape).

    • View popup
    Table 3.

    Growth of Xylella fastidiosa isolates elm and grape growth in periwinkle wilt broth supplemented with either oxytetracycline (antibiotic) or paclobutrazol (PBZ).z

    Treatment and doseMean log CFU/mL−1
    ElmGrape
    Oxytetracycline 50 μg/mL−10.15 a2.19 a
    PBZ 200 μg/mL−12.66 b3.64 b
    PBZ 20 μg/mL−17.35 c7.48 c
    PBZ 2 μg/mL−17.33 c7.67 c
    Periwinkle wilt media7.61 c7.98 c
    • ↵zGrowth was determined by dilution plating.

    • Values are means of three replicates after 10 days of incubation.

    • Means in each column with identical letters are not significantly different at the 0.05 level by the Tukey test SED = 0.040 (elm), 0.037 (grape).

    • CFU = colony-forming unit.

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Arboriculture & Urban Forestry (AUF): 33 (4)
Arboriculture & Urban Forestry (AUF)
Vol. 33, Issue 4
July 2007
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Effect of the Growth Regulator Paclobutrazol on Growth of the Bacterial Pathogen Xylella fastidiosa
Darren A. DeStefano, Arv P. Grybauskas, James L. Sherald, Bahram Momen, Qi Huang, Joe H. Sullivan
Arboriculture & Urban Forestry (AUF) Jul 2007, 33 (4) 246-252; DOI: 10.48044/jauf.2007.028

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Effect of the Growth Regulator Paclobutrazol on Growth of the Bacterial Pathogen Xylella fastidiosa
Darren A. DeStefano, Arv P. Grybauskas, James L. Sherald, Bahram Momen, Qi Huang, Joe H. Sullivan
Arboriculture & Urban Forestry (AUF) Jul 2007, 33 (4) 246-252; DOI: 10.48044/jauf.2007.028
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