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Research ArticleArticles

Growth and Iron Sequestering of Pin Oak (Quercus palustris) Seedlings Inoculated with Soil Containing Ectomycorrhizal Fungi

Richard J. Hauer and Jeffrey O. Dawson
Arboriculture & Urban Forestry (AUF) May 1996, 22 (3) 122-130; DOI: https://doi.org/10.48044/jauf.1996.019
Richard J. Hauer
Graduate Research Assistant and Professor of Tree Physiology, Department of Natural Resources and Environmental Sciences, University of Illinois, Urbana, IL 61801
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Jeffrey O. Dawson
Graduate Research Assistant and Professor of Tree Physiology, Department of Natural Resources and Environmental Sciences, University of Illinois, Urbana, IL 61801
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Article Figures & Data

Tables

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    Table 1.

    The ectomycorrhizal infection of lateral roots of pin oak (Quercus palustris) seedlings grown in either acidic or alkaline medium and inoculated with live or autoclaved soil from 3 different sites.

    Soil pH1Site2Autoclaved inoculum% lateral root infection
    AcidicForestNo77.7 ab3
    AcidicChloroticNo88.9 ab
    AcidicNon-chloroticNo87.2 ab
    AcidicForestYes0.0 c
    AcidicChloroticYes0.0 c
    AcidicNon-chloroticYes1.7c
    AlkalineForestNo77.3 ab
    AlkalineChloroticNo71.4 b
    AlkalineNon-chloroticNo96.6 a
    AlkalineForestYes0.0 c
    AlkalineChloroticYes0.0 c
    AlkalineNon-chloroticYes0.0 c
    ANALYSIS OF VARIANCE
    Model0.00014
    Ph0.7367
    Site0.4220
    Inoculation0.0001
    pH*Site0.5778
    pH*lnoculation0.8215
    Site*lnoculation0.5073
    pH*Site*lnoculation0.5066
    • ↵1 Acidic soil pH = 5.5 and alkaline soil pH = 7.5.

    • ↵2 Soil inoculum from pin oak forest stand, urban chlorotic pin oak trees, and urban non-chlorotic pin oak trees.

    • ↵3 Means (n = 36) in the same column with a different letter are significantly different at the α = 0.05 level using a Fisher’s protected LSD.

    • ↵4 Significance probabilities from the F statistic from an ANOVA. Significant values (P ≤ 0.05) in bold.

    • View popup
    Table 2.

    Biomass partitioning and root to shoot ratio of 7-month-old pin oak (Quercus palustris) seedlings grown in either acidic or alkaline soil medium and inoculated with live or autoclaved soil from 3 different sites.

    Soil pH1Site2Autoclaved inoculumShoot mass(g)Root mass(g)Total mass(g)Root/shoot
    AcidicForestNo1.63 a5.32 ab36.97 a3.23 a
    AcidicChloroticNo1.73 a5.31 ab7.06 a3.01 a
    AcidicNon-chloroticNo2.00 a5.57 a7.60 a2.99 a
    AcidicForestYes1.13 a3.09 cd4.27 a2.62 a
    AcidicChloroticYes1.73 a3.33 bcd5.09 a2.03 a
    AcidicNon-chloroticYes2.03 a4.68 abc6.77 a2.31 a
    AlkalineForestNo1.13 a2.15 d3.34 a1.92 a
    AlkalineChloroticNo1.53 a3.41 bcd4.98 a2.22 a
    AlkalineNon-chloroticNo1.73 a4.40 abc6.18 a2.47 a
    AlkalineForestYes1.37 a3.28 bcd4.69 a2.22 a
    AlkalineChloroticYes1.57 a4.17 abcd5.80 a2.66 a
    AlkalineNon-chloroticYes1.57 a4.54 abc6.16 a2.76 a
    Means1.604.105.742.54
    ANALYSIS OF VARIANCE
    Model0.405740.04590.06080.4138
    PH0.16160.03760.04110.1718
    Site0.04110.04150.02330.8376
    Inoculation0.70180.22220.28340.3782
    pH*Site0.81830.58210.76130.2738
    pH*lnoculation0.55490.00740.01960.0247
    Site*lnoculation0.92730.97050.97980.9796
    pH*Site*lnoculation0.46520.49140.43770.8844
    • ↵1 Acidic soil pH = 5.5 and alkaline soil pH = 7.5.

    • ↵2 Soil inoculum from pin oak forest stand, urban chlorotic pin oak trees, and urban non-chlorotic pin oak trees.

    • ↵3 Means (n = 36) in the same column with a different letter are significantly different at the α = 0.05 level using a Fisher’s protected LSD.

    • ↵4 Significance probabilities from the F statistic from an ANOVA. Significant values (P ≤ 0.05) in bold.

    • View popup
    Table 3.

    Biomass partitioning and root to shoot ratio of 2-year-old pin oak (Quercus palustris) seedlings grown in either acidic or alkaline soil medium and inoculated with live or autoclaved soil from 3 different sites.

    Soil pH1Site2Autoclaved inoculumShoot mass(g)Root mass(g)Total mass(g)Root/shoot
    AcidicForestNo7.83 a38.41 ab16.23 a1.09 a
    AcidicChloroticNo5.83 ab7.92 abc13.76 ab1.36 a
    AcidicNon-chloroticNo7.46 a8.24 ab15.69 a1.13 a
    AcidicForestYes7.94 a8.43 ab16.37 a1.05 a
    AcidicChloroticYes5.12 abcd6.95 abc12.07 ab1.39 a
    AcidicNon-chloroticYes7.19 a10.33 a17.51 a1.47 a
    AlkalineForestNo5.60 abc7.50 abc13.09 ab1.35 a
    AlkalineChloroticNo3.36 bcd5.47 bcd8.83 bc1.72 a
    AlkalineNon-chloroticNo5.60 abc6.96 abc12.55 ab1.46 a
    AlkalineForestYes2.19 d3.24 d5.43 c1.50 a
    AlkalineChloroticYes3.79 bcd4.79 cd8.49 bc1.35 a
    AlkalineNon-chloroticYes2.65 cd3.17 d5.82 c1.10 a
    Means5.386.7812.151.33
    ANALYSIS OF VARIANCE
    Model0.001240.00210.00100.0545
    pH0.00010.00010.00010.0541
    Site0.14210.53930.29360.1089
    Inoculation0.06580.06870.05500.6066
    pH*Site0.36500.53290.51570.1914
    pH*lnoculation0.16800.01990.04720.0761
    Site*lnoculation0.50980.68610.65510.5092
    pH*Site*lnoculation0.24940.18130.19430.0887
    • ↵1 Acidic soil pH = 5.5 and alkaline soil pH = 7.5.

    • ↵2 Soil inoculum from pin oak forest stand, urban chlorotic pin oak trees, and urban non-chlorotic pin oak trees.

    • ↵3 Means (n = 36) in the same column with a different letter are significantly different at the α = 0.05 level using a Fisher’s protected LSD.

    • ↵4 Significance probabilities from the F statistic from an ANOVA. Significant values (P ≤ 0.05) in bold.

    • View popup
    Table 4.

    The concentration of iron in leaf tissue from 2-year-old pin oak (Quercus palustris) seedlings grown in either acidic or alkaline soil medium and inoculated with live or autoclaved soil from 3 different sites.

    Soil pH1Site2Autoclaved inoculumIron (ppm)
    AcidicForestNo53.1 abcde3
    AcidicChloroticNo48.7 bcde
    AcidicNon-chloroticNo46.1 cde
    AcidicForestYes54.1 abcd
    AcidicChloroticYes65.0 a
    AcidicNon-chloroticYes58.7 ab
    AlkalineForestNo56.1 abc
    AlkalineChloroticNo55.2 abc
    AlkalineNon-chloroticNo46.7 bcde
    AlkalineForestYes51.3 bcde
    AlkalineChloroticYes40.7 e
    AlkalineNon-chloroticYes42.5 de
    Means5.15
    ANALYSIS OF VARIANCE
    Model0.01374
    Ph0.0336
    Site0.2365
    Inoculation0.6769
    pH*Site0.2998
    pH*lnoculation0.0010
    Site*lnoculation0.6136
    pH*Site*lnoculation0.1450
    • ↵1 Acidic soil pH = 5.5 and alkaline soil pH = 7.5.

    • ↵2 Soil inoculum from pin oak forest stand, urban chlorotic pin oak trees, and urban non-chlorotic pin oak trees.

    • ↵3 Means (n = 36) in the same column with a different letter are significantly different at the α = 0.05 level using a Fisher’s protected LSD.

    • ↵4 Significance probabilities from the F statistic from an ANOVA. Significant values (P ≤ 0.05) in bold.

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Arboriculture & Urban Forestry (AUF)
Vol. 22, Issue 3
May 1996
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Growth and Iron Sequestering of Pin Oak (Quercus palustris) Seedlings Inoculated with Soil Containing Ectomycorrhizal Fungi
Richard J. Hauer, Jeffrey O. Dawson
Arboriculture & Urban Forestry (AUF) May 1996, 22 (3) 122-130; DOI: 10.48044/jauf.1996.019

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Growth and Iron Sequestering of Pin Oak (Quercus palustris) Seedlings Inoculated with Soil Containing Ectomycorrhizal Fungi
Richard J. Hauer, Jeffrey O. Dawson
Arboriculture & Urban Forestry (AUF) May 1996, 22 (3) 122-130; DOI: 10.48044/jauf.1996.019
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