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Research ArticleArticles

Managing Wound-Associated Diseases by Understanding Wound Healing in the Bark of Woody Plants

Alan R. Biggs
Arboriculture & Urban Forestry (AUF) May 1990, 16 (5) 108-112; DOI: https://doi.org/10.48044/jauf.1990.029
Alan R. Biggs
West Virginia University, University Experiment Farm, P.O. Box 609, Kearneysville, WV 25430
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    Table 1. Estimates for numbers of days for wound responses of peach bark to occur following hypothetical injuries made in March, April, May, and June in Vineland, Ontario.z
    Injury dateNumber of days
    Lignin formationSuberin formation
    March 154962
    March 313649
    April 152739
    April 302232
    May 1 51827
    May 311523
    June 151321
    • ↵z Based on averaged annual degree-day accumulations (base = O C) in Vineland Station, Ontario, from 1935-1985. Estimated accumulated degree-days required for complete lignification and complete suberization are 256 and 411, respectively.

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    Table 2. Lignin-thioglycolic acid (LTGA) extracted from bark of peach pruning wounds 14 days after treatment with various substances.
    TreatmentLTGA (mg/g)yLTGA (Percent of control)
    Fungal wall extract (FWE)31.0 az+ 81.3
    Cellobiose (0.3 M)29.2 a+ 70.8
    Plant wall extract (PWE) + FWE23.2 ab+ 35.6
    Ca2+ (15 M)22.8 ab+ 33:4
    Glutathione (3.25 mM)20.6 ab+ 20.7
    PWE20.3 ab+ 18.7
    Chitosan + Ca2+ (1 5 M)20.3 ab+ 18.6
    Ca2+ (150 M)18.8 ab+ 9.7
    Control (wrapped)17.1 bc0.0
    Ethrel (7.0 mM)15.1 bcd- 11.8
    Chitosan (20 g/ml)15.1 bcd- 11.8
    GA3 + IAA (100 M + 100 M)14.9 bcd- 12.5
    Abscissic acid (100 M)14.8 bcd- 13.3
    Lac Balsam11.0 cd- 35.7
    White latex paint8.6 d- 49.6
    Control (nonwrapped)3.2 d- 81.2
    • ↵y Each value is the mean of nine observations except for control (treated with water and wrapped with parafilm (n = 48) and control (nonwrapped, exposed to the environment) (n = 15).

    • ↵z Letters denote significant differences determined with single degree-of-freedom orthogonal comparisons (P < 0.05).

    • View popup
    Table 3. Suberin autofluorescence (mV) from bark of peach pruning wounds 14 days after treatment with various substances.
    TreatmentSuberin autofluorescenceyPercent of control
    Control (nonwrapped)7 A az+ 33.4
    Chitosan + Ca2+ (1 5 M)7.4 a+ 33.1
    Fungal wall extract (FWE)7.3 a+ 32.2
    Ethrel (7.0 mM)6.9 a+ 25.0
    Abscissic acid (100 M)6.8 ab+ 23.6
    Glutathione (3.25 mM)5.8 abc+ 4.3
    Plant wall extract (PWE)5.6 abc+ 1.0
    Control (wrapped)5.5 abc0.0
    Chitosan (20 g/ml)5.3 abc- 3.8
    Cellobiose (0.3 M)5.0 abc- 9.8
    GA" + IAA (100 M + 100 M)4.2 bed- 24.4
    Ca2+ (150 M)4.2 bed- 24.9
    White latex paint4.0 cd- 26.8
    PWE + FWE3.9 cd- 29.6
    Ca2+(15 M)3.7 cd- 33.7
    Lac balsam2.0 d- 64.2
    • ↵y Each value is the mean of six observations except for control (treated with water and wrapped with parafilm) (n = 38) and control (nonwrapped, exposed to the environment (n = 37).

    • ↵z Letter denote significant difference determined with single degree-of-freedom orthogonal comparison (P < 0.05).

    • View popup
    Table 4. Percent infection and canker length 7 days postinoculation from twigs pre-treated with various substancesy
    Treatment groupCanker length (mm)Percent infection
    Control23.4 az66.7 b
    BAj + IAA16.4 b82.5 a
    LPWE12.9 be48.5 c
    LCWE11.2 cd74.7 ab
    Cellobiose10.8 cd47.4 c
    Calcium6.6 d21.0 d
    • ↵y Values in both columns are the means of 50 observations except for control (n = 10) and GA3 + IAA (n = 40). LPWE and LOWE are cell wall extracts from Leucostoma persoonii and L. cincta, respectively.

    • ↵z Letters denote significant differences determined with Duncan’s multiple range test (P < 0.05).

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Arboriculture & Urban Forestry (AUF)
Vol. 16, Issue 5
May 1990
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Managing Wound-Associated Diseases by Understanding Wound Healing in the Bark of Woody Plants
Alan R. Biggs
Arboriculture & Urban Forestry (AUF) May 1990, 16 (5) 108-112; DOI: 10.48044/jauf.1990.029

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Managing Wound-Associated Diseases by Understanding Wound Healing in the Bark of Woody Plants
Alan R. Biggs
Arboriculture & Urban Forestry (AUF) May 1990, 16 (5) 108-112; DOI: 10.48044/jauf.1990.029
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  • Article
    • Abstract
    • What Happens in Bark After Wounding?
    • Is Wound Response Related to Disease Resistance?
    • How Does Environment Influence Wound Response?
    • How Do Host Genetics Influence Wound Response?
    • Genetic Variation Within Peach
    • Can Wound Response Be Stimulated by Chemical Applications?
    • Summary
    • Footnotes
    • Literature Cited
  • Figures & Data
  • Info & Metrics
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